SAML Response Validation Fails

I am having an issue with the Validate function. We are able to decrypt the assertions, but the Validate function returns false. I have tried to validate the same response on (https://www.samltool.com/validate_response.php) as a test, and the request validates assuming the Idp EntityId and the SP EntityId are filled in correctly. Is there a setting on the validate function that will allow us to pass that information? Thanks.